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automacs running buffer  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec automacs running buffer
    Automacs Running Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 371 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/macs+separation/autoMACS+Running+Buffer+-+MACS+Separation+Buffer/us12692546-503-17-20
    Average 97 stars, based on 371 article reviews
    automacs running buffer - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Isolation:

    Article Title: Exosomal POU5 F1 derived from TNBC promotes cancer progression by regulating M2 macrophage polarization via inhibiting TRAF6 ubiquitination and activating AKT in macrophage.
    Article Snippet: After washing, the cells were treated with anti-FITC microbeads (Miltenyi Biotec) for 20 min to facilitate magnetic separation. .. Finally, the F4/80+ macrophage population was isolated by MACS separation following the manufacturer’s guidelines (Miltenyi Biotec). .. Statistical analysis All experimental data are conducted using GraphPad Prism 9.

    Article Title: Tumor‐Associated Glycan Exploits Adenosine Receptor 2A Signaling to Facilitate Immune Evasion
    Article Snippet: .. PBMCs were isolated from heparinized blood using Ficoll‐Paque Plus density gradient centrifugation (17‐144‐002, Cytiva), and CD4 + T cells were isolated by MACS separation (130‐097‐048, Miltenyi Biotech) according to the manufacturer's protocol. ..

    Article Title: Virus-like antigen display delivers a stand–alone danger signal through the BCR that circumvents tolerance
    Article Snippet: .. CD4+ T cells were isolated from the spleen and lymph nodes of OT-II mice utilizing MACS separation and the CD4+ T cell negative selection kit (Miltenyi, see above). ..

    Magnetic Cell Separation:

    Article Title: Exosomal POU5 F1 derived from TNBC promotes cancer progression by regulating M2 macrophage polarization via inhibiting TRAF6 ubiquitination and activating AKT in macrophage.
    Article Snippet: After washing, the cells were treated with anti-FITC microbeads (Miltenyi Biotec) for 20 min to facilitate magnetic separation. .. Finally, the F4/80+ macrophage population was isolated by MACS separation following the manufacturer’s guidelines (Miltenyi Biotec). .. Statistical analysis All experimental data are conducted using GraphPad Prism 9.

    Article Title: Sex differences in DNMT3A-mutant clonal hematopoiesis and the effects of estrogen.
    Article Snippet: .. Samples for single-cell sequencing experiments were enriched for c-kit+ cells via MACS separation using CD117 MicroBeads (Miltenyi Biotec; RRID:AB_2753213). ..

    Article Title: Intratumoral expression of JAML on NK cells is controlled by tumor microenvironment and MHC class I interaction
    Article Snippet: .. Single-cell suspension from spleens was depleted of erythrocytes, and NK cells were positively sorted by negative sorting using MACS separation, according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Cells were resuspended in complete medium (MEMalpha; 10 mM HEPES, 2 × 10 −5 M 2-ME, 10% FCS, 100 U/ml penicillin, 100 U/ml streptomycin) with 1000U/ml IL-2 (RNDsystems).

    Article Title: The cytoplasmic scaffolding protein PCNA regulates NLRP3 inflammasome activation in macrophages
    Article Snippet: .. Hoechst 33342 solution was from ThermoScientific. μMACS Protein A Microbeads 130-071-001), MACS Separation μ Columns 130-042-701) and μMACSTM Separator were from Miltenyi Biotec. ..

    Article Title: The cardiac phospho-proteome during pressure overload in mice.
    Article Snippet: .. For endothelial cell isolation, the pellet was resuspended in MACS buffer, incubated with CD146 microbeads (Miltenyi Biotec, 130-092-007) (20 μL per sample), and subjected to MACS separation using MS columns (Miltenyi Biotec, MS columns, 130-042-201) on a magnetic stand. ..

    Article Title: Mitochondrial protein translation is a heightened dependence and therapeutic vulnerability of chemo-refractory triple negative breast cancer
    Article Snippet: .. MACS separation (Miltenyi Biotec 130-104-694) was performed according to the manufacturer’s protocol. .. The final cell count of the enriched human cells was obtained, and 5,000 cells were plated per 50 μl in growth medium in 96-well low-attachment plates (Corning 3474).

    Article Title: Tumor‐Associated Glycan Exploits Adenosine Receptor 2A Signaling to Facilitate Immune Evasion
    Article Snippet: .. PBMCs were isolated from heparinized blood using Ficoll‐Paque Plus density gradient centrifugation (17‐144‐002, Cytiva), and CD4 + T cells were isolated by MACS separation (130‐097‐048, Miltenyi Biotech) according to the manufacturer's protocol. ..

    Article Title: Virus-like antigen display delivers a stand–alone danger signal through the BCR that circumvents tolerance
    Article Snippet: .. CD4+ T cells were isolated from the spleen and lymph nodes of OT-II mice utilizing MACS separation and the CD4+ T cell negative selection kit (Miltenyi, see above). ..

    Single Cell:

    Article Title: Sex differences in DNMT3A-mutant clonal hematopoiesis and the effects of estrogen.
    Article Snippet: .. Samples for single-cell sequencing experiments were enriched for c-kit+ cells via MACS separation using CD117 MicroBeads (Miltenyi Biotec; RRID:AB_2753213). ..

    Article Title: Intratumoral expression of JAML on NK cells is controlled by tumor microenvironment and MHC class I interaction
    Article Snippet: .. Single-cell suspension from spleens was depleted of erythrocytes, and NK cells were positively sorted by negative sorting using MACS separation, according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Cells were resuspended in complete medium (MEMalpha; 10 mM HEPES, 2 × 10 −5 M 2-ME, 10% FCS, 100 U/ml penicillin, 100 U/ml streptomycin) with 1000U/ml IL-2 (RNDsystems).

    Sequencing:

    Article Title: Sex differences in DNMT3A-mutant clonal hematopoiesis and the effects of estrogen.
    Article Snippet: .. Samples for single-cell sequencing experiments were enriched for c-kit+ cells via MACS separation using CD117 MicroBeads (Miltenyi Biotec; RRID:AB_2753213). ..

    Suspension:

    Article Title: Intratumoral expression of JAML on NK cells is controlled by tumor microenvironment and MHC class I interaction
    Article Snippet: .. Single-cell suspension from spleens was depleted of erythrocytes, and NK cells were positively sorted by negative sorting using MACS separation, according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Cells were resuspended in complete medium (MEMalpha; 10 mM HEPES, 2 × 10 −5 M 2-ME, 10% FCS, 100 U/ml penicillin, 100 U/ml streptomycin) with 1000U/ml IL-2 (RNDsystems).

    Cell Isolation:

    Article Title: The cardiac phospho-proteome during pressure overload in mice.
    Article Snippet: .. For endothelial cell isolation, the pellet was resuspended in MACS buffer, incubated with CD146 microbeads (Miltenyi Biotec, 130-092-007) (20 μL per sample), and subjected to MACS separation using MS columns (Miltenyi Biotec, MS columns, 130-042-201) on a magnetic stand. ..

    Incubation:

    Article Title: The cardiac phospho-proteome during pressure overload in mice.
    Article Snippet: .. For endothelial cell isolation, the pellet was resuspended in MACS buffer, incubated with CD146 microbeads (Miltenyi Biotec, 130-092-007) (20 μL per sample), and subjected to MACS separation using MS columns (Miltenyi Biotec, MS columns, 130-042-201) on a magnetic stand. ..

    Gradient Centrifugation:

    Article Title: Tumor‐Associated Glycan Exploits Adenosine Receptor 2A Signaling to Facilitate Immune Evasion
    Article Snippet: .. PBMCs were isolated from heparinized blood using Ficoll‐Paque Plus density gradient centrifugation (17‐144‐002, Cytiva), and CD4 + T cells were isolated by MACS separation (130‐097‐048, Miltenyi Biotech) according to the manufacturer's protocol. ..

    Selection:

    Article Title: Virus-like antigen display delivers a stand–alone danger signal through the BCR that circumvents tolerance
    Article Snippet: .. CD4+ T cells were isolated from the spleen and lymph nodes of OT-II mice utilizing MACS separation and the CD4+ T cell negative selection kit (Miltenyi, see above). ..



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